Regulatory

Part:BBa_K2084003:Design

Designed by: Aife Ni Chochlain   Group: iGEM16_Pittsburgh   (2016-10-12)


pT7 Toehold Regulated T3 RNAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2694


Design Notes

na


Source

The T3 RNAP is from the T3 bacteriophage. The toehold is completely synthetic and from the Collins Lab at MIT. The T7 promoter is from the T7 bacteriophage.

References